By Johanna Maria Ticar
The grasp thesis of Johanna Maria Ticar finds high-resolution insights into the myocardial microstructure and illustrates that cardiac muscle fibers are instantly, working in parallel with one most well-liked fiber path, even though, deposits akin to fats appear to compromise the standard and compact constitution. moment harmonic iteration imaging mixed with optical tissue clearing is a correct procedure for picking out the 3-dimensional muscle fiber and sheet orientations and as a result, permits the calculation of fiber rotation in the course of the ventricle wall.
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Extra resources for 3D Analysis of the Myocardial Microstructure: Determination of Fiber and Sheet Orientations
7 depicts two different samples, both having a depth of 500 μm with a step size of 5 μm in between individual slices and in-plane dimension 620×620 μm. 14) is higher. 1 as this sample was considered to be an outlier. Although both samples have b-values above 1 (indicating highly aligned ﬁbers), the ﬁber alignment of the sample shown in (a) is superior. The comparatively increased dispersion parameter κ of the image stack in (b) indicates a more isotropic ﬁber distribution. One can observe the better arrangment of ﬁbers in (a) as the muscle ﬁbers run more straight and parallel than in (b).
This deposit embedded in the heart muscle ﬁbers modiﬁes the ﬁber orientation. While (a) demonstrates the deposit, the z-stack in (b) which was rotated approximately 90◦ in order to display the yz-plane, additionally visualizes the formation of cleavage planes. 3: Volume rendering of optical sections obtained via SHG imaging. (a) and (b) show the same z-stack featuring a depth of 640 μm, but from distinct perspectives. 2 Multi-photon microscopy 33 The z-stack shown in Fig. 4 was opened to show the details of the muscle ﬁbers on two opposing surfaces.
Hence, blue areas indicate the presence of only few to no ﬁbers. 2). From the ﬁt, both the location parameter μ and the concentration parameter b were obtained and calculated for every single image in the z-stack. The location parameter μ describes the principal (preferred) ﬁber orientation of the distribution, while the concentration parameter b determines the shape of the von Mises distribution. A narrower distribution indicates elevated b-values and hence, more aligned ﬁbers. Therefore, a more anisotropic ﬁber distribution follows.